Mitochondrial import inner membrane translocase subunit TIM23 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P32897 (TIM23_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 109. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Mitochondrial import inner membrane translocase subunit TIM23

Alternative name(s):
Membrane import machinery protein MIM23
Mitochondrial protein import protein 3
Mitochondrial protein import protein MAS6

Gene names

Name:	TIM23
Synonyms:	MAS6, MIM23, MPI3
Ordered Locus Names:	YNR017W
ORF Names:	N3180

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	222 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.
Subunit structure	Component of the TIM23 complex, at least composed of TIM23, TIM17, TIM50 and TIM21. The complex interacts with the TIM44 component of the PAM complex. Ref.8
Subcellular location	Mitochondrion inner membrane; Multi-pass membrane protein. Note: Around 20 amino acids of the N-terminus are believed to span the mitochondrial outer membrane. This association is dynamic and depends on the translocation activity of the TIM23 complex. However, this topology seems not to be critical for formation of a TOM-TIM supercomplex and preprotein import. Ref.9
Miscellaneous	Present with 1440 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the Tim17/Tim22/Tim23 family.

Ontologies

Keywords
Biological process	Protein transport Translocation Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein import into mitochondrial matrix Inferred from mutant phenotype Ref.6 PubMed 7600576. Source: SGD
Cellular_component	integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD mitochondrial inner membrane presequence translocase complex Inferred from physical interaction PubMed 7600576. Source: SGD mitochondrial intermembrane space Traceable author statement. Source: Reactome
Molecular_function	P-P-bond-hydrolysis-driven protein transmembrane transporter activity Inferred from electronic annotation. Source: InterPro mitochondrion targeting sequence binding Inferred from direct assay PubMed 8858146. Source: SGD protein channel activity Inferred from direct assay PubMed 11713477. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
TIM17	P39515	5	EBI-9136,EBI-9127
TIM21	P53220	5	EBI-9136,EBI-23128
TIM44	Q01852	3	EBI-9136,EBI-9141
TIM50	Q02776	2	EBI-9136,EBI-30302

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 222

222

Mitochondrial import inner membrane translocase subunit TIM23

PRO_0000210307

Regions

Transmembrane

1 – ?

Helical; Note=Outer membrane; Potential

Topological domain

? – 96

Mitochondrial intermembrane Potential

Transmembrane

97 – 118

Helical; Note=Inner membrane; Potential

Topological domain

119 – 144

Mitochondrial matrix Potential

Transmembrane

145 – 166

Helical; Note=Inner membrane; Potential

Topological domain

167 – 174

Mitochondrial intermembrane Potential

Transmembrane

175 – 189

Helical; Note=Inner membrane; Potential

Topological domain

190 – 196

Mitochondrial matrix Potential

Transmembrane

197 – 215

Helical; Note=Inner membrane; Potential

Topological domain

216 – 222

Mitochondrial intermembrane Potential

Sequences

Sequence

Length

Mass (Da)

Tools

P32897 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 08E5AB4F400F675B
Show »

FASTA

222

23,244

        10         20         30         40         50         60 
MSWLFGDKTP TDDANAAVGG QDTTKPKELS LKQSLGFEPN INNIISGPGG MHVDTARLHP 

        70         80         90        100        110        120 
LAGLDKGVEY LDLEEEQLSS LEGSQGLIPS RGWTDDLCYG TGAVYLLGLG IGGFSGMMQG 

       130        140        150        160        170        180 
LQNIPPNSPG KLQLNTVLNH ITKRGPFLGN NAGILALSYN IINSTIDALR GKHDTAGSIG 

       190        200        210        220 
AGALTGALFK SSKGLKPMGY SSAMVAAACA VWCSVKKRLL EK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"MAS6 encodes an essential inner membrane component of the yeast mitochondrial protein import pathway." Emtage J.L.T., Jensen R.E. J. Cell Biol. 122:1003-1012(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Identification of MIM23, a putative component of the protein import machinery of the mitochondrial inner membrane." Dekker P.J.T., Keil P., Rassow J., Maarse A.C., Pfanner N., Meijer M. FEBS Lett. 330:66-70(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"The polytopic mitochondrial inner membrane proteins MIM17 and MIM23 operate at the same preprotein import site." Kuebrich M., Keil P., Rassow J., Dekker P.J.T., Blom J., Meijer M., Pfanner N. FEBS Lett. 349:222-228(1994) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION, TOPOLOGY.
[6]	"Tim23 links the inner and outer mitochondrial membranes." Donzeau M., Kaldi K., Adam A., Paschen S., Wanner G., Guiard B., Bauer M.F., Neupert W., Brunner M. Cell 101:401-412(2000) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Mitochondrial presequence translocase: switching between TOM tethering and motor recruitment involves Tim21 and Tim17." Chacinska A., Lind M., Frazier A.E., Dudek J., Meisinger C., Geissler A., Sickmann A., Meyer H.E., Truscott K.N., Guiard B., Pfanner N., Rehling P. Cell 120:817-829(2005) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE TIM23 COMPLEX.
[9]	"Active remodelling of the TIM23 complex during translocation of preproteins into mitochondria." Popov-Celeketic D., Mapa K., Neupert W., Mokranjac D. EMBO J. 27:1469-1480(2008) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, TOPOLOGY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X71633 Genomic DNA. Translation: CAA50640.1. X74161 Genomic DNA. Translation: CAA52274.1. Z71632 Genomic DNA. Translation: CAA96296.1. BK006947 Genomic DNA. Translation: DAA10558.1.
PIR	S36139.
RefSeq	NP_014414.3. NM_001183194.3.
3D structure databases
ProteinModelPortal	P32897.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2451N.
IntAct	P32897. 8 interactions.
MINT	MINT-1359281.
STRING	4932.YNR017W.
Protein family/group databases
TCDB	3.A.8.1.1. mitochondrial protein translocase (MPT) family.
Proteomic databases
PaxDb	P32897.
PeptideAtlas	P32897.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNR017W; YNR017W; YNR017W.
GeneID	855751.
KEGG	sce:YNR017W. sce:YNR024W.
Organism-specific databases
CYGD	YNR017w.
SGD	S000005300. TIM23.
Phylogenomic databases
eggNOG	COG5596.
GeneTree	ENSGT00390000001094.
HOGENOM	HOG000166435.
KO	K12594.
OMA	SDDLCYG.
OrthoDB	EOG4BP4N0.
Enzyme and pathway databases
Reactome	REACT_118590. Mitochondrial Protein Import (yeast). REACT_85873. Metabolism of proteins.
Gene expression databases
Genevestigator	P32897.
GermOnline	YNR017W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003397. Tim17/Tim22/Tim23/PMP24. IPR005681. Tim23. [Graphical view]
Pfam	PF02466. Tim17. 1 hit. [Graphical view]
TIGRFAMs	TIGR00983. 3a0801s02tim23. 1 hit.
ProtoNet	Search...
Other
BindingDB	P32897.
ChEMBL	CHEMBL1741180.
NextBio	980166.

Entry information

Entry name

TIM23_YEAST

Accession

Primary (citable) accession number: P32897
Secondary accession number(s): D6W1J2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	May 1, 2013
This is version 109 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents