Conserved oligomeric Golgi complex subunit 2 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Contribute

Send feedback

Read comments (?) or add your own

P53271 (COG2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 100. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Conserved oligomeric Golgi complex subunit 2
Short name=COG complex subunit 2

Alternative name(s):
Component of oligomeric Golgi complex 2
Protein SEC35

Gene names

Name:	COG2
Synonyms:	SEC35
Ordered Locus Names:	YGR120C
ORF Names:	G6324

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	262 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as component of the peripheral membrane COG complex that is involved in intra-Golgi protein trafficking. COG is located at the cis-Golgi, and regulates tethering of retrograde intra-Golgi vesicles and possibly a number of other membrane trafficking events. COG2 is required for ER to Golgi vesicle docking. Not essential for viability. Ref.4 Ref.7
Subunit structure	Component of the conserved oligomeric Golgi (COG or Sec34/Sec35) complex which consists of eight different proteins COG1-COG8. The COG complex interacts with the Rab GTPase YPT1, the Glogi SNAREs GOS1, SEC22, SED5, VTI1 and YKT6 and the COPI coatomer subunit gamma SEC21. Ref.5 Ref.6 Ref.7 Ref.11
Subcellular location	Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side Ref.4 Ref.9.
Miscellaneous	Present with 3270 molecules/cell in log phase SD medium.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Golgi apparatus Membrane
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	CVT pathway Inferred from mutant phenotype PubMed 20065092. Source: SGD ER to Golgi vesicle-mediated transport Inferred from genetic interaction PubMed 10512869. Source: SGD macroautophagy Inferred from mutant phenotype PubMed 20065092. Source: SGD peroxisome degradation Inferred from mutant phenotype PubMed 20065092. Source: SGD retrograde transport, vesicle recycling within Golgi Inferred from mutant phenotype Ref.5. Source: SGD
Cellular_component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell Golgi transport complex Inferred from physical interaction Ref.5. Source: SGD extrinsic to membrane Inferred from direct assay PubMed 10512869. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
COG1	P53079	8	EBI-16614,EBI-4835
COG3	P40094	12	EBI-16614,EBI-16605
COG4	Q06096	11	EBI-16614,EBI-4823
COG5	P53951	3	EBI-16614,EBI-4841
COG6	P53959	3	EBI-16614,EBI-4829
COG7	P53195	2	EBI-16614,EBI-4847
COG8	Q04632	4	EBI-16614,EBI-6035
SED5	Q01590	2	EBI-16614,EBI-16930

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 262

262

Conserved oligomeric Golgi complex subunit 2

PRO_0000213499

Secondary structure

262

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P53271 [UniParc].

Last modified July 11, 2006. Version 2.
Checksum: 0ED457A7466F0F53
Show »

FASTA

262

30,325

        10         20         30         40         50         60 
MDFLNDDELD LDLPVTAEIS KELFATEIEK YRESETNGTD VDNFDVDRFL VQKNFHYLPL 

        70         80         90        100        110        120 
DSLIRDLSGL SQKMVQTLLE QIRSNYDDYL TFSNTYTDEE NETLINLEKT QSDLQKFMTQ 

       130        140        150        160        170        180 
LDHLIKDDIS NTQEIIKDVL EYLKKLDEIY GSLRNHSQLT EALSLGKRLS KSLHEMCGIE 

       190        200        210        220        230        240 
PLEEEICSGL IEQLYKLITA SRRILESCAD SNSPYIHHLR NDYQDLLQEF QISLKILTEK 

       250        260 
CLENPSSLQN LSLTLVSIIK TA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An 18.3 kb DNA fragment from yeast chromosome VII carries four unknown open reading frames, the gene for an Asn synthase, remnants of Ty and three tRNA genes." van Dyck L., Tettelin H., Purnelle B., Goffeau A. Yeast 13:171-176(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Sec35p, a novel peripheral membrane protein, is required for ER to Golgi vesicle docking." VanRheenen S.M., Cao X., Lupashin V.V., Barlowe C., Waters M.G. J. Cell Biol. 141:1107-1119(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[5]	"The Sec34/35 Golgi transport complex is related to the exocyst, defining a family of complexes involved in multiple steps of membrane traffic." Whyte J.R., Munro S. Dev. Cell 1:527-537(2001) [PubMed] [Europe PMC] [Abstract] Cited for: SUBUNIT.
[6]	"The Sec34/Sec35p complex, a Ypt1p effector required for retrograde intra-Golgi trafficking, interacts with Golgi SNAREs and COPI vesicle coat proteins." Suvorova E.S., Duden R., Lupashin V.V. J. Cell Biol. 157:631-643(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN COG COMPLEX, SUBUNIT.
[7]	"Identification of sec36p, sec37p, and sec38p: components of yeast complex that contains sec34p and sec35p." Ram R.J., Li B., Kaiser C.A. Mol. Biol. Cell 13:1484-1500(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE COG COMPLEX.
[8]	"Sequencing and comparison of yeast species to identify genes and regulatory elements." Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S. Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
[9]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[10]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]	"The binary interacting network of the conserved oligomeric Golgi tethering complex." Loh E., Hong W. J. Biol. Chem. 279:24640-24648(2004) [PubMed] [Europe PMC] [Abstract] Cited for: COMPOSITION OF THE COG COMPLEX, INTERACTION WITH COG3 AND COG4.
[12]	"Structural analysis of conserved oligomeric Golgi complex subunit 2." Cavanaugh L.F., Chen X., Richardson B.C., Ungar D., Pelczer I., Rizo J., Hughson F.M. J. Biol. Chem. 282:23418-23426(2007) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 61-262.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X83099 Genomic DNA. Translation: CAA58155.1.
Z72905 Genomic DNA. Translation: CAA97130.1.
BK006941 Genomic DNA. Translation: DAA08212.1.

PIR

S55978.

RefSeq

NP_011635.2. NM_001181249.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2JQQ	NMR	-	A	61-262	[»]

ProteinModelPortal

P53271.

SMR

P53271. Positions 109-262.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-1194N.

IntAct

P53271. 29 interactions.

MINT

MINT-376961.

STRING

4932.YGR120C.

Proteomic databases

PaxDb

P53271.

PeptideAtlas

P53271.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YGR120C; YGR120C; YGR120C.

GeneID

853017.

KEGG

sce:YGR120C.

Organism-specific databases

CYGD

YGR120c.

SGD

S000003352. COG2.

Phylogenomic databases

eggNOG

NOG41082.

HOGENOM

HOG000111879.

OMA

RESETNG.

OrthoDB

EOG4JT3G0.

Gene expression databases

Genevestigator

P53271.

GermOnline

YGR120C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR024602. COG_su2_N.
[Graphical view]

Pfam

PF06148. COG2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P53271.

NextBio

972884.

Entry information

Entry name

COG2_YEAST

Accession

Primary (citable) accession number: P53271
Secondary accession number(s): D6VUQ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	July 11, 2006
Last modified:	April 3, 2013
This is version 100 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents