Q02776 (TIM50_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitochondrial import inner membrane translocase subunit TIM50

Gene names

Name:	TIM50
Ordered Locus Names:	YPL063W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	476 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Required to direct preproteins in transit and direct them to the channel protein TIM23, and possibly facilitates transfer of the translocating proteins from the TOM complex to the TIM23 complex. Does not act as a structural subunit of the TIM23 complex but plays a dynamic role in protein import. Has no phosphatase activity. Ref.3 Ref.4 Ref.5
Subunit structure	Component of the TIM23 complex, at least composed of TIM23, TIM17, TIM50 and TIM21. Interacts directly with TIM23. Interacts with preproteins in transit. Ref.3 Ref.4 Ref.8
Subcellular location	Mitochondrion inner membrane; Single-pass membrane protein Ref.3 Ref.4 Ref.6.
Domain	The mitochondrial intermembrane region is required for generation but not stabilization of the TIM23 complex. Ref.5 The FCP1 homology domain does not contain the canonical D-x-D-x-[TV] active site, suggesting that it probably does not display phosphatase activity. Ref.5
Miscellaneous	Present with 2400 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the TIM50 family. Contains 1 FCP1 homology domain.

Ontologies

Keywords
Biological process	Protein transport Translocation Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein import into mitochondrial matrix Inferred from mutant phenotype Ref.3Ref.4. Source: SGD
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial inner membrane presequence translocase complex Inferred from direct assay Ref.3Ref.4. Source: SGD
Molecular_function	mitochondrion targeting sequence binding Inferred from direct assay PubMed 18418384PubMed 19144822. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TIM23	P32897	2	EBI-30302,EBI-9136

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 43	43	Mitochondrion Probable
	Chain	44 – 476	433	Mitochondrial import inner membrane translocase subunit TIM50		PRO_0000043139
Regions
	Topological domain	44 – 114	71	Mitochondrial matrix Potential
	Transmembrane	115 – 131	17	Helical; Potential
	Topological domain	132 – 476	345	Mitochondrial intermembrane Potential
	Domain	187 – 330	144	FCP1 homology

Sequences

	Sequence		Length	Mass (Da)
	Q02776 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 778D54F5C4ED27C8	FASTA	476	55,099
	10 20 30 40 50 60 MLSILRNSVR LNSRALRVVP SAANTLTSVQ ASRRLLTSYS SFLQKETKDD KPKSILTDDM 70 80 90 100 110 120 LFKAGVDVDE KGQGKNEETS GEGGEDKNEP SSKSEKSRRK RQTSTDIKRE KYANWFYIFS 130 140 150 160 170 180 LSALTGTAIY MARDWEPQES EELKKDIDNG YTLSLMYKRF KARFNSMFTY FQEPPFPDLL 190 200 210 220 230 240 PPPPPPPYQR PLTLVITLED FLVHSEWSQK HGWRTAKRPG ADYFLGYLSQ YYEIVLFSSN 250 260 270 280 290 300 YMMYSDKIAE KLDPIHAFVS YNLFKEHCVY KDGVHIKDLS KLNRDLSKVI IIDTDPNSYK 310 320 330 340 350 360 LQPENAIPME PWNGEADDKL VRLIPFLEYL ATQQTKDVRP ILNSFEDKKN LAEEFDHRVK 370 380 390 400 410 420 KLKDKFYGDH KSGGNWAMTA LGLGNSLGGS TKFPLDLIHE EGQKNYLMFM KMIEEEKEKI 430 440 450 460 470 RIQQEQMGGQ TFTLKDYVEG NLPSPEEQMK IQLEKQKEVD ALFEEEKKKK KIAESK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"The mitochondrial presequence translocase: an essential role of Tim50 in directing preproteins to the import channel." Geissler A., Chacinska A., Truscott K.N., Wiedemann N., Brandner K., Sickmann A., Meyer H.E., Meisinger C., Pfanner N., Rehling P. Cell 111:507-518(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE TIM23 COMPLEX, PROBABLE TRANSIT PEPTIDE SITE, LACK OF PHOSPHATSE ACTIVITY, FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, INTERACTION WITH TIM23.
[4]	"Tim50 is a subunit of the TIM23 complex that links protein translocation across the outer and inner mitochondrial membranes." Yamamoto H., Esaki M., Kanamori T., Tamura Y., Nishikawa S., Endo T. Cell 111:519-528(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE TIM23 COMPLEX, PROBABLE TRANSIT PEPTIDE SITE, FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, INTERACTION WITH TIM23.
[5]	"Mitochondrial translocation contact sites: separation of dynamic and stabilizing elements in formation of a TOM-TIM-preprotein supercomplex." Chacinska A., Rehling P., Guiard B., Frazier A.E., Schulze-Specking A., Pfanner N., Voos W., Meisinger C. EMBO J. 22:5370-5381(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DOMAIN INTERMEMBRANE.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Mitochondrial presequence translocase: switching between TOM tethering and motor recruitment involves Tim21 and Tim17." Chacinska A., Lind M., Frazier A.E., Dudek J., Meisinger C., Geissler A., Sickmann A., Meyer H.E., Truscott K.N., Guiard B., Pfanner N., Rehling P. Cell 120:817-829(2005) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE TIM23 COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U39205 Genomic DNA. Translation: AAB68302.1. BK006949 Genomic DNA. Translation: DAA11368.1.
PIR	S60927.
RefSeq	NP_015262.1. NM_001183877.1.
3D structure databases
ProteinModelPortal	Q02776.
SMR	Q02776. Positions 195-361.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-8544N.
IntAct	Q02776. 13 interactions.
MINT	MINT-3374965.
STRING	4932.YPL063W.
Protein family/group databases
TCDB	3.A.8.1.1. mitochondrial protein translocase (MPT) family.
Proteomic databases
PaxDb	Q02776.
PeptideAtlas	Q02776.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPL063W; YPL063W; YPL063W.
GeneID	856042.
KEGG	sce:YPL063W.
Organism-specific databases
CYGD	YPL063w.
SGD	S000005984. TIM50.
Phylogenomic databases
eggNOG	COG5190.
GeneTree	ENSGT00550000075036.
HOGENOM	HOG000183658.
OMA	GYLSQYY.
OrthoDB	EOG4R53BJ.
Enzyme and pathway databases
Reactome	REACT_118590. Mitochondrial Protein Import (yeast). REACT_85873. Metabolism of proteins.
Gene expression databases
Genevestigator	Q02776.
GermOnline	YPL063W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.40.50.1000. 1 hit.
InterPro	IPR023214. HAD-like_dom. IPR004274. NIF. [Graphical view]
Pfam	PF03031. NIF. 1 hit. [Graphical view]
SMART	SM00577. CPDc. 1 hit. [Graphical view]
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
PROSITE	PS50969. FCP1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	980986.

Entry information

Entry name

TIM50_YEAST

Accession

Primary (citable) accession number: Q02776
Secondary accession number(s): D6W3V2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program